2017-06-25 12:22:452025-05-10 06:59:45
title
yvcK
glmR
description
essential for growth under gluconeogenetic conditions, control of cell shape, required for correct localization of [[protein|PonA|PBP1]]
regulator of carbon partitioning between central metabolism and peptidoglycan biosynthesis, control of [SW|cell shape], required for correct localization of [[protein|PonA|PBP1]]
locus
BSU34760
BSU_34760
geneLength
951
954
function
localization of [[protein|PonA|PBP1]]
regulation of carbon partitioning between central metabolism and peptidoglycan biosynthesis, localization of [[protein|PonA|PBP1]]
product
unknown
activator of [[protein|GlmS]] activity
names
yvcK
glmR
yvcK
outlinks
bsu
BSU34760
BSU_34760
Gene
Coordinates
3,570,546 → 3,571,499
3,570,546 3,571,499
Gene
Phenotypes of a mutant
unable to grow with gluconeogenic substrates as single carbon source [Pubmed|16272399]
filamentous or L-shape-like aberrant morphologies (suppressed by Mg2+) [Pubmed|16272399], this is supressed by overexpression of [[protein|MreB]] or by deletion of [[gene|ponA|PBP1]] [Pubmed|21320184]
unable to grow with gluconeogenic substrates as single carbon source, this can be suppressed by mutations in [[gene|cggR]], [[gene|zwf]], [[gene|pgpH]], [[gene|ltaS]], [[gene|yfnI]], upon overexpression of [[gene|glmS]] or by a point mutation in [[protein|PgcA]] (G47S) that results in increased phosphoglucosamine mutase activity [Pubmed|31589605,30478337,30248093,16272399]
filamentous or L-shape-like aberrant morphologies (suppressed by Mg2+) [Pubmed|16272399], this is supressed by overexpression of [[protein|MreB]] or by deletion of [[gene|ponA|PBP1]], [[gene|ltaS]], or [[gene|yfnI]] [Pubmed|30478337,21320184]
a [[gene|pgcA]] [[gene|glmR]] double mutant is not viable, this can be suppressed by overexpression of [[protein|GlmM]] [pubmed|31589605]
The protein
Protein family
UPF0052 family (according to Swiss-Prot)
gluconeogenesis factor family (single member, according to UniProt)
The protein
Effectors of protein activity
binds UDP-sugars (UDP-Glc, UDP-GlcNAc) [pubmed|28646159]
binds UDP-GlcNAc [pubmed|28646159], this prevents the interaction with [[protein|GlmS]] and stimulates interaction with [[protein|YvcJ]] [pubmed|32994436,30248093]
Biological materials
Mutant
MGNA-B640 (yvcK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1639 NBRP B. subtilis, Japan]
MGNA-B640 (yvcK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1639 NBRP B. subtilis, Japan]
BKE34760 (Δ[[gene|glmR]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGCGATTTTCGGCTTTTGTC, downstream forward: _UP4_TGAAGCCTTGAAATGAGGTG
BKK34760 (Δ[[gene|glmR]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34760 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TGCGATTTTCGGCTTTTGTC, downstream forward: _UP4_TGAAGCCTTGAAATGAGGTG
Biological materials
Expression vector
pGP736 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Stülke] lab
Biological materials
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW| Görke] lab
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Boris Görke]'s lab
Labs working on this gene/protein
[SW|Anne Galinier], University of Marseille, France
References
Original publications
The protein
Catalyzed reaction/ biological activity
stimulates [[protein|GlmS]] activity to facilitate the diversion of carbon from fructose-6-phosphate to peptidoglycan synthesis [pubmed|32994436,30248093]
interacts with [[protein|YvcJ]] to facilitate genetic transformation [pubmed|32994436]
The protein
Paralogous protein(s)
[[this]]
Biological materials
Expression vectors
pGP736 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab
labs
[SW|Anne Galinier], University of Marseille, France